To investigate the potential relevance of loss of NME1 for human breast cancer, we analyzed NME1 transcript levels by RTqPCR and NME1 protein levels by Western blotting of a panel of human breast cell lines according to their normal-like, estrogen receptor (ER)-positive, and triple-negative (hormone receptors negative) status (Figure 9). Here, NME1 is linked to breast cancer.