As a first step to investigate the contributions of NME1 and NME2 to EMT, we depleted a human breast carcinoma cell line, MCF10DCIS.com [36], which has an epithelial-like phenotype, specifically of NME1 or NME2 by expressing siRNAs and then measured expression of EMT-related proteins including E-cadherin, cytokeratin 18, β-catenin, N-cadherin, and vimentin by Western blotting (Figure 1). Here, NME1 is linked to breast carcinoma.