Real-time reverse transcription-PCR (RT-PCR) results showed that the expression of IL-1β mRNA was significantly induced in S. pneumoniae-infected macrophages of WT and NLRP6−/− mice at 9 h post infection (Figure 2A), indicating that NLRP6 did not affect S. pneumoniae-induced IL-1β transcription. Here, IL1B is linked to infection.