With the cell model of mouse melanoma B16-F10 cells, we also revealed the anti-melanogenic capabilities of OLM and its different fractions by mechanisms involving the decrease in tyrosinase and TRP-2 expressions and melanin productions via downregulating MITF and p-CREB and differentially upregulating p-ERK or p-JNK. The gene discussed is TYR; the disease is melanoma.