Third, we used immunohistochemical (IHC) staining of FN, VIM, and TGF-β in the specimens isolated from the same patient received more than two times surgeries (origin and recurrence) to evaluate the correlation between FN expression and TGF-β-induced EMT in the malignant glioma patients with local recurrence, remote brain, or spinal metastasis. Here, FN1 is linked to malignant glioma.