CD40LG and leptospirosis: Several methods are available for the detection of leptospirosis that include polymerase chain reaction (PCR), multiplex loop-mediated isothermal amplification (m-LAMP), Immunoglobulin-M (IgM)-based enzyme-linked immunosorbent assay (IgM ELISA), and the microscopic agglutination test (MAT) [3,4].