To corroborate these findings, we performed an immunoassay (MultiPlex) to address the serum content of different growth factors and cytokines associated with unfavorable prognosis in HCC, such as EGF, FGF-2, Follistatin, HGF and VEGF-A [30,33,34,35]. This evidence concerns the gene FGF2 and hepatocellular carcinoma.