The AML cell lines MOLM-13 (AML FAB M5a) and MV4-11 (AML FAB M5) were treated with VPA (0, 0.5, 1 and 2 mM) for 48 and 72 h, followed by 2D gel immunoblot analysis with an antibody against p53 (Bp53-12; detects p53 FL, p53β and p53γ) (Figure 3A), or 1D gel immunoblot analysis using antibodies against p53 (DO-12; detects all p53 isoforms), p53 (Bp53-12), p21 and actin (Figure 3B). Here, TP53 is linked to acute myeloid leukemia.