Based on the results obtained for the specific binding assay, we were able to further determine that the non-specific binding represents 18% of the total binding, and the remaining 52% is attributed to the specific binding of neurotensin to its receptors (NTRS1, NTRS2 and NTRS3), In the case of 177Lu-DOTA-NT, 5 h after cell incubation, the cell retention profile follows a sudden decreasing trend of radioactivity, which might signify the colon cancer cells death and detachment from the Petri dish. Here, NTS is linked to colonic neoplasm.