The link between SPINK1 and increasing sphere formation ability in prostate cancer was also validated using the 22RV1 cell line and by regulating the expression of genes involved in stemness and epithelial-mesenchymal transition (EMT), including SNAI1 (SNAIL), SNAI2 (SLUG), and TWIST1 [36]. Here, SPINK1 is linked to Familial prostate cancer.