CISD2 and Wilson disease: To test whether the genetic dosage of Cisd2 modulates the severity of pathogenesis of WD-induced fatty liver, mice having different copy numbers of Cisd2 in their livers and thus expressing different levels of Cisd2 protein (Figure 1A), namely Cisd2hKO+/− (50%), WT (100%), and Cisd2TG (200%), were fed the WD for 4 months.