Furthermore, the immunoprecipitation analysis of Complex I subunit NDUFB8 demonstrated the reduction of O-GlcNAcylated levels (Figure 8B,D; * p < 0.05, CTR vs. IPA: −15%) after IPA treatment, as previously observed in HFD mice, confirming the strong correlation between insulin resistance-induced mitochondrial defects and impaired O-GlcNAcylation profile. The gene discussed is NDUFB8; the disease is Insulin resistance.