AKT1 and cancer: With respect to HT-29 cancer cells, treatment with KA39 at a TGI concentration of 15.9 μΜ for 3 and 24 h induced significant inhibition of Akt1 phosphorylation, decreasing the Akt1 phospho-form by 72.9% and 88.5%, respectively (p < 0.01) (Figure 3A).