SRC and colorectal carcinoma: Targeted Sanger sequencing for exon 5 of the SRC gene confirmed pedigree segregation of the prioritized variant, showing the heterozygous mutation SRCV177M in two family members (III-1, IV-8) with CRC and in the possible carrier (IV-7) and the wild-type sequence in two family members without CRC, for whom the DNA samples were available and tested by Sanger sequencing (IV-9, IV-10, Figure 2b).