Of interest, acetyl-CoA can be produced by PDH from glucose and by acyl-coenzyme A synthetase short-chain family member 2 (ACSS2) from acetate, both of which are closely associated with the biology of the malignant brain tumor, glioblastoma (GBM), through acetylation of cytosolic proteins (Figure 2) [81]. Here, ACSS2 is linked to glioblastoma.