First, we intended to compare the contents of the incubation media obtained after this compound treatment for three different colon tumor cell lines: (i) HCT-116, which is naturally characterized by a low level of UGT expression; (ii) HCT-116 stably transfected with the UGT1A10 gene and (iii) HT-29, with naturally high expression of UGT enzymes. Here, UGT1A10 is linked to colonic neoplasm.