Furthermore, an immunofluorescence assay was conducted to investigate the expression and localization of ADAMTS1 in granulosa cells, and the results showed that ADAMTS1 was mainly expressed in the cytoplasm of granulosa cells, and the staining intensity of ADAMTS1 in granulosa cells from PCOS patients was stronger as compared with the ovulatory controls (Figure 1C). Here, ADAMTS1 is linked to polycystic ovary syndrome.