Analysis of the TCGA HNSCC cohort revealed that 14, namely, NOP2, NSUN2, NSUN3, NSUN4, NSUN5, NSUN6, NSUN7, DNMT1, DNMT3A, DNMT3B, TET2, TET3, ALYREF, and YBX1, out of 15 m5C-related RNA regulators exhibited different expression hallmarks among tumors and normal controls. This evidence concerns the gene DNMT3B and head and neck squamous cell carcinoma.