TARDBP and proteostasis deficiencies: Since TDP-43 delocalization to the cytoplasm, and its consequent depletion from the nucleus, is a prominent pathological feature in ALS and related TDP-43 proteinopathies (Prasad et al., 2019), we then evaluated by confocal microscopy the cell distribution of overexpressed (WT or Q331K) TDP-43-mKate2 in HEK293T cells, in the absence or the presence of overexpressed NCL-GFP.