To date, most HBV transfection experiments have been based on Huh7 cells, due to much greater transfection efficiency, but stable transfection of HepG2 rather than Huh7 cells with sodium taurocholate cotransporting polypeptide (NTCP) enables efficient infection by cell culture-derived HBV particles (69, 80, 81); that should enable the characterization of the biological properties of HBV genetic variants by both transient transfection and infection experiments in HepG2/NTCP cells. This evidence concerns the gene SLC10A1 and infection.