Considering the natural history of malignancy progression of malignancy from AGII to GBM, an upregulation of GLSiso2, GOT2, and GPT2 expression levels were observed, in contrast to the downregulation of GLUD1. However, a large spreading of their expressions was detected in GBM, consistent with the well-known heterogeneity observed in GBM. This evidence concerns the gene GLUD1 and glioblastoma.