These dysregulated genes were mainly involved in metabolism pathways as shown by KEGG analysis, including fatty acid metabolism, pyruvate metabolism, metabolic pathways, fatty acid biosynthesis, AMPK signalling pathway, biosynthesis of unsaturated fatty acids, insulin signaling pathway, propanoate metabolism, and others, (Figure 4C), indicating the potential role of CHRDL1 in regulating metabolism status in PCOS patients. This evidence concerns the gene INS and polycystic ovary syndrome.