The tumor growth delay assay in Figure 1F–1H (Control group, yellow line; Chemotherapy group, black line; anti-PD-1 group, red line; Combined group, green line), for MC38-hPD-L1 tumor, tumor growth was inhibited in both combined treatment group and anti-PD-1 treated alone group, compared with the 5-FU+OX alone group (Figure 1F, Combined vs. Chemotherapy group, P<0.001; anti-PD-1 group vs. Chemotherapy, P<0.001). This evidence concerns the gene PDCD1 and neoplasm.