We used RT-qPCR technology to detect the relative expression of the ULBP2 gene in COAD tumor tissues and adjacent normal tissues, and the results indicated that the relative expression was significantly higher in COAD tumor tissues (0.23233246±0.48443959501) than in adjacent normal tissues (0.02814684 ± 0.037491460) (P = 0.012; Figure 4A, 4B). This evidence concerns the gene ULBP2 and neoplasm.