Consistent with the reporter assays, qRT-PCR results revealed when miR-155 was overexpressed (Figure 2E(Fig. 2)), SMAD5 and TGF-βR2 mRNA expression levels were significantly lower compared to siCtrl in CD34+ CML cells (Figure 2F, G(Fig. 2)). The gene discussed is SMAD5; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.