Because Nek2 is altered in breast cancers by overexpression and/or gene amplification, we tested if the overexpression of GFP-Nek2 is sufficient to trigger CA and binucleation in MCF10A, an established, p53-wild-type, a non-transformed mammary epithelial cell line with low percentages of CA and CIN22,23,36,43,72. This evidence concerns the gene TP53 and breast carcinoma.