In this research, we reported that SIRT1 knockout also upregulated the protein levels of TGF-β (p < 0.001) and pSMAD3 (p < 0.01) in PD mice, indicating that SIRT1 deficiency activated the TGF-β/SMAD3 pathway and enhanced peritoneal fibrosis. This evidence concerns the gene SMAD3 and Peritoneal Fibrosis.