Tumor size (HR: 2.332, 95%CI: 1.650–3.296, P < 0.001), Tumor number (HR: 2.198, 95%CI: 1.551–3.114, P < 0.001), AFP (HR: 1.452, 95%CI: 1.010–2.087, P = 0.044), vascular invasion (HR: 2.095, 95%CI: 1.485–2.955, P < 0.001), BCLC stage (HR: 2.893, 95%CI: 1.932–4.332, P < 0.001), GGT (HR: 2.084, 95%CI: 1.431–3.036, P < 0.001), and coNLR-PDW (HR: 2.292, 95%CI: 1.737–3.024, P < 0.001) are significantly associated with DFS according to univariate regression analysis. The gene discussed is AFP; the disease is neoplasm.