The presence of advanced liver fibrosis (S3-S4) was associated with AST, ALP, TBil, DBil, cholinesterase, albumin, globulin, prealbumin, total bile acid, TC, TG, LDL, prothrombin time, INR, thrombin time, RBC, platelet count, neutrophil count, hyaluronic acid, cholylglycine, laminin, PIIINP, and type IV collagen. The gene discussed is TTR; the disease is Hepatic fibrosis.