LMNB1 and infection: In order to prove that nuclear HIV-1 complexes had entered through intact nuclear pores and did not enter during nuclear envelope breakdown in cells that had escaped cell cycle arrest under our conditions (Figure 4—figure supplement 1e,f), we observed eBFP2.LMNB1 expressing TZM-bl cells over the whole 12 hr time course of infection by live imaging (Figure 4l and m, Figure 4—video 1) before PF74 treatment and IF staining.