Infection of an eGFP.OR3 expressing SupT1 cell line (Figure 7—figure supplement 1) or of primary activated CD4+ T cells transduced to express eGFP.OR3 (Figure 7a) with NNHIV ANCH showed nuclear OR3 punctae and separation of IN-FP and OR3 punctae as observed for TZM-bl cells. Here, CD4 is linked to infection.