The Tconv/Treg ratio, however, was significantly increased in distant tumors of the ZD55-IL-24 therapy alone, anti-PD-1 therapy alone and combination therapy groups (Supplementary Figure 2a, c), suggesting that both ZD55-IL-24 treatment and anti-PD-1 treatment can also elicit remodeling of the immunosuppressive tumor microenvironment in distant tumors with contributions to the immunotherapy of distant tumors. The gene discussed is PDCD1; the disease is neoplasm.