Consistent with the tumor growth data, the survival of mice receiving ZD55-IL-24 plus anti-PD-1 therapy was significantly prolonged compared with other treatments, although the ZD55-IL-24 monotherapy and ZD55-IL-24 plus isotype IgG therapy were also able to greatly prolong the shortened lifespan of mice (Fig. 3d). This evidence concerns the gene PDCD1 and neoplasm.