AKT1 and myelodysplastic syndrome: Analyzing the significantly differentially methylated genes leading to the clusters, we found that the majority of genes were hypermethylated in cluster A compared to cluster B. Among those genes, there were some interesting candidates whose aberrant methylation had been associated with inferior outcome in MDS in prior studies, e.g., WT1 [21], DLX6 (Distal-Less Homeobox 6) [24], members of the PI3K-Akt-mTOR and MAPK pathways [14] and, importantly, members of the Wnt signaling pathway.