To explore the detailed cellular impacts of VIM-AS1 on bladder carcinoma metastasis, we knock down VIM-AS1 in the high-metastatic bladder cancer cell line T24 by transfection of si1-VIM-AS1 or si2-VIM-AS1 (Fig. 2a), and overexpressed VIM-AS1 in the low-metastatic bladder carcinoma cell RT24 by transfection of the VIM-AS1-overexpressing vector (Fig. 2f), as confirmed by real-time PCR. The gene discussed is VIM-AS1; the disease is urinary bladder cancer.