At the end of the experiment (the 25th day), mice were euthanized and tumor tissues were excised, the tumor volumes, weight and sizes of xenograft formed by T24 cells with VIM-AS1 overexpression were clearly greater than those formed by normal T24 cells, while the tumor volumes, weight and sizes in Lv-miR-655 group were observably smaller than normal group; VIM-AS1 overexpression could relieves the inhibition effect of miR-655 on the growth and tumorigenesis of bladder cancer (Fig. 5a). This evidence concerns the gene VIM and urinary bladder cancer.