The duration of detectability of anti-DENV IgM antibody is not well defined, including how it may differ by infecting DENV serotype, status of primary versus secondary DENV infection, and symptomatic versus asymptomatic infection.35 Also, given the period for which participants were asked questions regarding symptoms and preventive behavior, recall bias may be a limitation for the accuracy and completeness of the collected data, especially for detailed information on frequency of using prevention methods. This evidence concerns the gene CD40LG and infection.