PLCG1 and neoplasm: Future studies are required to elucidate how prednisolone alters hESF CM and thus decidual regulation of trophoblast invasion, however, the data presented here suggests hESF CM may alter trophoblast motility genes including PLCG1. PLCG1 has not previously been identified in trophoblast or the placenta, however, it has well established roles in tumor metastasis where it promotes cell invasion (Kunze et al., 2014; Jang et al., 2018; Tang et al., 2019), potentially via its interactions with MMP2 (Zhang et al., 2019) or EGFR signaling (Jang et al., 2018).