Consistently, the inhibition of autophagic response by siBeclin1 and siATG5 significantly enhanced CPT-induced apoptosis, as best evidenced by the increase of Annexin V-positive cell populations (Figures 2C, D) and the accumulation of cleaved PARP, a classical marker of apoptosis (Figures 2E, F) in esophageal cancer cells. Here, ANXA5 is linked to esophageal cancer.