It is worth noting that even though we employed FACS and sorted the cells dissociated from the primary CRC tumors to enrich CSCs by commonly used CSC surface markers, the proportion of CSCs was still low (≈3.9%) in the whole cell population, and LGR5+ cells were still heterogeneous (Figure S1d, Supporting Information), further highlighting the importance and necessity of single‐cell analysis, which presumably can capture the unique features of rare CSCs in a tumor. This evidence concerns the gene LGR5 and neoplasm.