Another clue that mechanisms other than AIF‐mediated caspase‐independent apoptosis coexist and underlie the proapoptotic function of OTUD1 is that although Z‐VAD treatment could not entirely suppress the proapoptotic role of OTUD1 on ESCC cells, it indeed showed some rescuing effects, which indicates that the caspase‐dependent apoptosis pathway is also involved in the proapoptotic functions of OTUD1 (Figure 2H,I; Figure S2F, Supporting Information). This evidence concerns the gene OTUD1 and esophageal squamous cell carcinoma.