AKT1 and hepatocellular carcinoma: Moreover, both the immunoblot analysis and immunochemistry staining demonstrated that the T308 phosphorylation of Akt was significantly increased not only in de novo sorafenib-resistant HCC cells and tissues (Fig, 1F, 1G & S1C) but also in acquired sorafenib-resistant HCC cells (Fig. 1H & S1D).