The gene ontology analysis on the common upregulated/downregulated genes between the two groups of HCC cells displayed that PI3K/Akt, focal adhesion, Neuroactive ligand/receptor, cytokine/cytokine receptor, and cAMP signaling pathways were mostly regulated in sorafenib-resistant cells (Fig. 1D). Here, AKT1 is linked to hepatocellular carcinoma.