In addition, the creation of a rapid bone metastasis model by intracardiac injections of MDA PCa 2b cells into nude mice revealed that MYBL2 overexpression increased the hindlimb tumor burden and the number of bone metastatic lesions, significantly accelerated the onset of bone metastasis, and markedly shortened the survival time in tumor-bearing mice (Figure 6I-K). This evidence concerns the gene MYBL2 and neoplasm.