Cell Counting Kit-8 (CCK8) and colony formation assays demonstrated that HOXB5 upregulation can increase HCC cell proliferation of PLC/PRF/5 cells, whereas HOXB5 knockdown impaired HCC cell proliferation of MHCC97H cells (Figure S2A and B). The gene discussed is HOXB5; the disease is hepatocellular carcinoma.