As shown in Figure 5E, the interaction (red dots) between Rac1 and YWHAZ was translocated into nuclei in HF-IR-treated mouse testis, while remained cytoplasmic in SB202190-treated cohorts, indicating a protein-protein co-localization between Rac1 and YWHAZ from cytoplasm (pre-IR) to nucleus (post-IR). This evidence concerns the gene RAC1 and hydrops fetalis.