To further study how miR-375 regulated the process of pancreatic differentiation of ESCs, we selected a variety of pathways from early pancreatic development to late insulin secretion-related pathways, such as the WNT signaling pathway (hsa04310) (157 genes), NOTCH signaling pathway (hsa04330) (52 genes), HEDGEHOG signaling pathway (hsa04340) (50 genes), and mature onset diabetes in young people (hsa04950) (including 26 genes) for further analysis. Here, INS is linked to type 2 diabetes mellitus.