To determine whether ALKBH6 protects pancreatic cancer cells from accumulating alkylating agent-induced DNA damage, we performed siRNA-mediated silencing of ALKBH6 in BXPC3 and MIA-PaCa-2 pancreatic cancer cell lines and performed an immunofluorescence co-localization assay. Here, ALKBH6 is linked to familial pancreatic carcinoma.