In agreement with previous reports, a recent study reported that a single injection of AAV-mediated FST administration after several weeks of HFD feeding induced browning of subcutaneous WAT by upregulation of PGC-1α, PRDM16, UCP1 and beige-specific CD137, and decreased obesity-associated metabolic inflammation (145). The gene discussed is UCP1; the disease is obesity due to melanocortin 4 receptor deficiency.