SIX3 and infection: After infection of primary dispersed human islet cells, pseudoislet development and subsequent purification of GFP+ cells (Methods), qRT-PCR revealed increased levels of both SIX2 and SIX3 mRNA after CRISPRa-SIXE compared to controls (Fig. 6C; n = 6 independent donors); the low abundance of SIX3-AS1 mRNA precluded detection in these studies.