For example, the variant rs1002226 falls within an enhancer hub in the KCNJ11-ABCC8 locus associated with diabetes risk, and we adapted our lentiCRISPR construct to simultaneously express two sgRNAs (sg_EK1 and sg_EK2) and induce a small deletion in the putative enhancer encompassing this risk SNP (Fig. 4A, B: Methods;35,39). Here, ABCC8 is linked to diabetes mellitus.