Eleven patients presented with decreased ctF ratio (ctF ratio: ctF2/ctF1, ctF1 refers to the highest AF in the first test, and ctF2 refers to the highest AF in the second test) (Figure 6, Supplementary Table 4), which is consistent with their imaging findings: 10 patients had significantly shrunken tumor size, and 1 patient had no significant increase in tumor size. This evidence concerns the gene CTF1 and neoplasm.