In addition, isolated EVs were co-cultured with LSCs to detect the expression pattens of miR-1246 and LRIG1 in LSCs, and the results showed that miR-1246 expression was significantly up-regulated, while LRIG1 expression was decreased in LSCs following co-culture with AML-EVs. The gene discussed is LRIG1; the disease is acute myeloid leukemia.