In this study, we developed a new blood-based early CRC screening test, which combined two previously reported methylation markers, c9orf50 and twist1, and two novel genes, kcnj12 and znf132. Methylated twist1 and c9orf50 have been studied as novel blood-based markers for CRC detection recently [17, 19, 20]. This evidence concerns the gene TWIST1 and colorectal carcinoma.