Consistently, the weight of the subcutaneous tumors formed by the ZBTB18 overexpressing HCT116 and HT29 cells was significantly lower than in the tumors formed by the corresponding control sublines (Fig. 3d and Additional file 1: Fig. S10d), collectively demonstrating that reintroduction of ZBTB18 into colon cancer cells significantly reduced their growth. The gene discussed is ZBTB18; the disease is malignant colon neoplasm.