In total, 27 enriched functional clusters of the DEGs were obtained (Fig. 2b), such as ‘Pathways in cancer’ (20 genes), ‘PI3K-Akt signaling pathway’ (18 genes), ‘Proteoglycans in cancer’ (15 genes), ‘MAPK signaling pathway’ 13 genes), ‘Regulation of actin cytoskeleton’ (13 genes). The gene discussed is AKT1; the disease is cancer.